In peptide synthesis, the quality of your reagents directly impacts the success of your experiments. Fmoc-Ala-OH (CAS 35661-39-3), a protected form of L-alanine, is no exception. Impurities in Fmoc-Ala-OH can lead to failed couplings, truncated peptides, and wasted resources. Whether you’re developing therapeutic peptides or conducting academic research, understanding CAS 35661-39-3 purity standards is essential.
This guide explains why purity matters, how to verify it, and why sourcing from trusted suppliers like Kilobio ensures consistent, high-quality results.
What is Fmoc-Ala-OH (CAS 35661-39-3)?
Fmoc-Ala-OH (9-Fluorenylmethoxycarbonyl-L-alanine) is a key reagent in solid-phase peptide synthesis (SPPS). The Fmoc group protects the amine terminus during chain assembly, enabling precise, stepwise elongation of peptides. Applications include:
- Drug development: Synthesizing peptide-based therapeutics.
- Biomaterials: Engineering bioactive surfaces.
- Academic research: Studying protein interactions.
With CAS 35661-39-3, Fmoc-Ala-OH is standardized for purity and stability. However, not all suppliers meet the stringent requirements for peptide synthesis.
Why Purity Matters in Fmoc-Ala-OH
Impure Fmoc-Ala-OH can introduce contaminants that:
- Reduce Coupling Efficiency: Impurities hinder peptide elongation, leading to truncated sequences.
- Compromise Yield: Side reactions or incomplete deprotection lower overall yield.
- Increase Costs: Failed experiments waste time, reagents, and resources.
For example, residual solvents like TFA (trifluoroacetic acid) or DMF (dimethylformamide) can interfere with synthesis. Similarly, oxidation or hydrolysis products can destabilize the reagent.
How to Verify Fmoc-Ala-OH Purity
1. Request a Certificate of Analysis (CoA)
A CoA provides detailed information about the reagent’s purity and quality. Look for:
- Purity: ≥98% by HPLC.
- Residual Solvents: <0.1% TFA or DMF.
- Water Content: <0.5% (critical for moisture-sensitive reagents).
Suppliers like Kilobio provide CoAs with every batch, ensuring transparency and compliance.
2. Perform In-House Testing
If possible, verify purity using:
- HPLC (High-Performance Liquid Chromatography): Detects impurities and quantifies purity.
- NMR (Nuclear Magnetic Resonance): Confirms molecular structure and identifies contaminants.
- Mass Spectrometry (MS): Detects trace impurities.
3. Check Supplier Certifications
Reputable suppliers adhere to:
- ISO 9001: Quality management standards.
- cGMP: Current Good Manufacturing Practices for pharmaceuticals.
- ISO 13485: Medical device quality management.
Common Impurities in Fmoc-Ala-OH
- Residual Solvents: TFA, DMF, or acetonitrile from synthesis.
- Oxidation Products: Degraded Fmoc groups or alanine side chains.
- Hydrolysis Products: Free alanine or Fmoc-OH from moisture exposure.
- Synthesis Byproducts: Unreacted intermediates or side products.
Why Choose High-Purity Fmoc-Ala-OH from Kilobio?
Kilobio is a trusted supplier of peptide synthesis reagents, offering:
- Rigorous QC Testing: Every batch undergoes HPLC, NMR, and MS analysis.
- Transparency: Instant access to CoAs for compliance and verification.
- Proper Packaging: Argon-flushed, desiccated vials to prevent degradation.
- Expert Support: Dedicated chemists assist with synthesis optimization.
Sample CoA Metrics:
- Purity: 99.2% (HPLC).
- Residual Solvents: <0.05% TFA.
- Water Content: 0.3%.
Submit an Inquiry: Contact Kilobio for pricing, bulk quotes, or custom synthesis requests.
FAQs: Fmoc-Ala-OH Purity
Q: What’s the minimum purity required for peptide synthesis?
A: ≥98% purity is standard, but ≥99% is recommended for critical applications.
Q: How do I store Fmoc-Ala-OH to maintain purity?
A: Store at -20°C in a desiccator to prevent moisture absorption and degradation.
Q: Can I use Fmoc-Ala-OH without a CoA?
A: It’s risky. Always request a CoA to verify purity and compliance.
The Risks of Low-Purity Fmoc-Ala-OH
Using subpar reagents can lead to:
- Failed Experiments: Impurities disrupt peptide elongation.
- Wasted Resources: Re-runs increase costs and delay timelines.
- Regulatory Non-Compliance: Lack of documentation can hinder approvals.
Suppliers like Kilobio ensure high-purity Fmoc-Ala-OH, minimizing these risks.
Final Recommendations
When sourcing Fmoc-Ala-OH (CAS 35661-39-3), prioritize suppliers that:
- Provide detailed CoAs.
- Adhere to ISO or cGMP standards.
- Offer proper packaging and storage guidance.
Ready to Order? Visit Kilobio’s Fmoc-Ala-OH Product Page to submit an inquiry or request a quote. Their team responds within 24 hours to assist with your needs.
