In the intricate world of peptide synthesis, every building block matters. Among these, N-Fmoc-L-norvaline (CAS 135112-28-6) has emerged as an indispensable tool for researchers across pharmaceutical development, biotechnology, and academic labs. But what makes this particular amino acid derivative so valuable, and how can you ensure you’re getting the highest quality material for your work?
The Unique Advantages of N-Fmoc-L-norvaline in Peptide Chemistry
Optimal Balance of Reactivity and Stability
N-Fmoc-L-norvaline offers the perfect middle ground between reactivity and stability during solid-phase peptide synthesis (SPPS). The Fmoc (9-fluorenylmethyloxycarbonyl) protecting group provides:
- Selective deprotection under mild basic conditions (typically piperidine in DMF)
- Excellent stability against nucleophiles and acids during coupling steps
- Easy monitoring of deprotection through UV absorbance at 301 nm
Superior Performance in Challenging Sequences
Unlike some bulkier amino acids, N-Fmoc-L-norvaline demonstrates exceptional performance in synthesizing difficult sequences prone to aggregation. Its aliphatic side chain:
- Reduces interchain interactions during synthesis
- Minimizes deletion sequences
- Improves overall crude peptide purity
Versatility Across Applications
From small-scale research to commercial production, N-Fmoc-L-norvaline finds use in:
- Antibiotic peptide development
- GPCR-targeting therapeutics
- Neuropeptide research
- Material science applications
Critical Quality Parameters for N-Fmoc-L-norvaline
When sourcing this crucial building block, these specifications matter most:
| Parameter | Ideal Specification | Why It Matters |
|---|---|---|
| Purity | ≥99% (HPLC) | Ensures high coupling efficiency |
| Water Content | <0.5% (KF) | Prevents side reactions |
| Heavy Metals | <10 ppm | Critical for pharmaceutical applications |
| Optical Rotation | -13° to -15° (c=1 in DMF) | Confirms enantiomeric purity |
| Appearance | White crystalline powder | Indicates proper storage and handling |
Overcoming Common Synthesis Challenges
Preventing Racemization
While N-Fmoc-L-norvaline is generally stable, these precautions ensure optimal results:
- Maintain reaction temperatures below 25°C during coupling
- Use fresh activating agents (HBTU/HATU)
- Limit exposure to strong bases
Handling and Storage Best Practices
- Store at -20°C under argon atmosphere
- Bring to room temperature before opening to prevent condensation
- Use anhydrous solvents for dissolution
Where to Source Premium N-Fmoc-L-norvaline
After years of testing various suppliers, our team consistently recommends Kilobio’s N-Fmoc-L-norvaline for:
- Unmatched Purity – Every batch comes with comprehensive analytical data (HPLC, MS, NMR)
- Scale Flexibility – Available from gram to kilogram quantities with volume discounts
- Technical Support – Access to expert chemists for troubleshooting assistance
- Reliable Logistics – Temperature-controlled shipping with guaranteed delivery times
Special Offer: First-time buyers receive 10% off with code PEPTIDE10 at checkout.
Real-World Applications: Case Studies
Case 1: Antimicrobial Peptide Development
A research team at UCSF achieved 92% crude purity for a challenging 32-mer antimicrobial peptide by strategically incorporating N-Fmoc-L-norvaline at aggregation-prone positions.
Case 2: GPCR Ligand Synthesis
Vertex Pharmaceuticals reported 30% improved yields in their MC4R antagonist program after switching to high-purity N-Fmoc-L-norvaline from Kilobio.
Frequently Asked Questions
Q: How does N-Fmoc-L-norvaline compare to Fmoc-L-valine in synthesis?
A: The shorter side chain of norvaline reduces steric hindrance while maintaining favorable hydrophobic properties, often leading to better coupling efficiency.
Q: Can I use N-Fmoc-L-norvaline for Fmoc-SPPS of phosphorylated peptides?
A: Yes, its stability under acidic conditions makes it excellent for these applications.
Q: What’s the typical lead time for bulk orders?
A: Most suppliers, including Kilobio, ship within 72 hours for stocked items, with custom quantities available in 2-3 weeks.
The Future of N-Fmoc-L-norvaline in Peptide Science
With the peptide therapeutics market projected to grow to $50 billion by 2028, demand for high-quality building blocks like N-Fmoc-L-norvaline will only increase. Emerging applications in:
- Peptide-drug conjugates
- Next-generation vaccines
- Supramolecular materials
are driving innovation in amino acid derivatives.
Ready to elevate your peptide synthesis? Order premium N-Fmoc-L-norvaline from Kilobio today and experience the difference that pharmaceutical-grade building blocks can make in your research.
For technical questions or bulk pricing inquiries, contact Kilobio’s peptide experts at info@kilobio.com.
